目的：本文旨在分析LINC01432基因6个位点多态性在内蒙古蒙古族雄激素性脱发中的差异。方法：本研究采用候选基因病例-对照的研究策略,收集蒙古族病例组39例、对照组44例。应用SNaPshot技术检测样本的6个SNPs位点在样本的基因分型,计算样本位点的HWE值、等位基因频率并进行基因型关联性分析、连锁不平衡分析、单体型分析。结果：单位点分析显示：蒙古族病例组和对照组位于LINC01432基因上的rs201571、rs1160312、rs6113491等位基因的关联性分析有统计学差异(P<0.05),其余位点的分析无统计学差异。连锁不平衡分析显示： LINC01432基因上rs6137444、rs2180439、rs1998076位点和rs201571、rs1160312、rs6113491位点均存在较强连锁性。单体型分析显示： LINC01432基因上的rs201571、rs1160312、rs6113491构成的单倍型TAA与增加雄激素性脱发的患病风险相关(OR=2.247,95%Cl=1.081-4.671,P=0.030),其余单倍型频率无统计学意义。结论： rs201571、rs1160312、rs6113491和内蒙古蒙古族雄激素性脱发存在关联性,rs201571、rs1160312和rs6113491多态性可能增加患雄激素性脱发风险。个体携带rs201571-rs1160312-rs6113491单倍型TAA可能增加内蒙古蒙古族雄激素性脱发的患病风险。

Objective:The purpose of this study was to analyze the difference of 6 SNPs polymorphism in androgenic alopecia of Mongolian nationality in Inner Mongolia. Methods:In this study, the candidate gene case-control research strategy was adopted, and androgen alopecia questionnaire and normal control questionnaire were designed uniformly. In the form of questionnaire and blood sampling, 39 cases of Mongolian case group and 44 cases of control group were collected. The experimental method is imldrtm multiple SNP typing method, multiple SNP typing kit to extract the whole blood genomic DNA of all samples, establish 8-plex SNPs complex amplification system, and use the technology of snapshot to detect the 6 SNPs loci in the sample genotyping, Plink 1.0.7 software, cgen3.8 software, GeneMapper 4.1 software, haploview software Software package and other relevant data statistical analysis of all samples of each site HWE value, allele frequency, genotype association analysis, linkage imbalance analysis, haplotype analysis.

内蒙古自治区自然科学基金项目，内蒙古自治区卫生健康科技计划项目