目的 探究不同方法鉴定包装饮用水中铜绿假单胞菌结果。方法 采用GB8538. 57—2016、API 20E鉴定及VITEK鉴定3种方法对25株野生型菌株进行鉴别,使用16SrRNA基因序列分析与3种鉴别结果进行比对。结果 GB8538. 57—2016鉴定结果与16SrRNA基因序列对比结果一致,API 20E鉴定5株为假阳性菌株,占比20.0%；VITEK鉴定3株为假阳性菌株,占比12.0%。结论 鉴定包装饮用水中铜绿假单胞菌需将GB8538. 57—2016、API 20E鉴定及VITEK鉴定互相结合,辅佐印证,提高鉴别准确性。

Abstract:Objective：ToexploretheidentificationofPseudomonasaeruginosainpackageddrinkingwaterbydifferent methods.Methods：25wild-typestrainswereidentifiedbyGB8538.57—2016，API20EandVITEK.16SrRNAGeneSequence analysiswasusedtocomparewiththreeidentificationresults.Results：TheidentificationresultsofGB8538.57—2016werecon- sistentwiththecomparisonresultsof16SrRNAgenesequence.FivestrainswereidentifiedasfalsepositivebyAPI20E，account- ingfor20.0%.ThreestrainswereidentifiedasfalsepositivebyVitek,accountingfor12.0%.Conclusion：Theidentificationof PseudomonasaeruginosainpackageddrinkingwatershouldbecombinedwithGB8538.57—2016，API20Eidentificationand VITEKidentification，assistinconfirmationandimprovetheaccuracyofidentification.