目的:建立蒙药齿缘草中槲皮素和山奈素含量的测定方法。 方法:采用高效液相色谱法。 色谱柱以 十八烷基硅烷键合硅胶为填充剂;流动相为甲醇~0.4%磷酸溶液(45颐 55);检测波长为368nm;流速为1.0mL/ min. 结果:槲皮素在31.4~314 ng 范围内有良好的线性关系(r =0.9997),平均回收率为96.96%,(RSD= 1.54%);山奈素在30~300ng范围内有良好的线性关系(r=0.9999),平均回收率为97.92%(RSD=1.91%)。 结论:该方法简便,准确,重复性好,可作为齿缘草中槲皮素和山奈素含量的测定方法。

Objective:To establish a determination method of quercetin and kaempferol in Eritrichii Herba. Methods:HPLCwas applied. Thecolumnwas Eighteenalkyl silanebondedsilicagel, themobilephasewas methanol ~0. 4%phosphoricacid(45颐 50),theflowratewas 1. 0mL/ minand thedetectionwavelengthwas 368nm. Result:The linear range of Quercetinwas 31. 4~314 ng(r= 0. 9997),theaveragerecoverywas96. 96%(RSD=1. 54%). Thelinear rangeof kaempferol was30~ 300ng(r=0. 9999),the average recovery was 97. 92%(RSD=1. 91%). Conclusion:The methodis simple,accurate,reproducible andcanbe usedfor the determinationof quercetinandkaempferol in Eritrichii Herba.

R917